Endocrine Abstracts

Oestrogen quantification in serum is challenging as concentrations are low, especially in men and post-menopausal women. Additionally, oestrogens do not ionise readily and structure similarities can lead to cross-reactivity and reduced specificity, especially with immunoassay. Full characterisation of oestrogen metabolism and its role in disease progression has therefore not been fully investigated. We aimed to develop and validate a liquid chromatography mass spectrometry met...

Gas chromatography–mass spectrometry (GC–MS) has been used for urinary steroid analysis for over 50 years. The process of hydrolysis to release steroids from their conjugates is routinely performed using commercially available snail Helix-Pomatia sulfatase (HP). However, as this is a naturally occurring enzyme its production process results in batch variation and so each batch requires experimental validation to adjust for the difference in efficiency of the enzymati...

Advancements in medical care have significantly improved survival rates following major traumatic injury. An understanding of the hormonal, inflammatory and metabolic changes that occur following trauma is still evolving but it is clear that they impact significantly upon patient prognosis. To date, studies that have examined trauma-induced changes in steroid metabolism have analysed samples taken from patients post-hospital admission, culminating in marked variability in the ...

Introduction: Melnick Needles Syndrome corresponds to a rare skeletal dysplasia of genetic etiology, with dominant inheritance pattern attached to the X chromosome. Mutations related to the gene of filamina A, which is responsible for the synthesis of collagen, are responsible for the clinical and phenotypic manifestations of the disease, with predominantly female involvement. Rickets are caused by inadequate mineralization of the bone, which precedes the closure of the epiphy...

Objectives: To evaluate the prevalence in our work center of pathology of adrenal glands and to assess the efficacy of the radiology imaging in the detection of especific features and to exclude malignancy lessions.Material and Methods: We included patients who presented some adrenal lesion with histological study after surgerie between January 2010 and December 2015 in the hospitals of Albacete, Almansa and Villarrobledo. We use the Pathological Anatomy...

Objective: Polycystic ovary syndrome (PCOS) is characterized by biochemical and clinical androgen excess and disturbed ovarian function and is associated with disorders of glucose and lipid metabolism. Isoflavones are phytoestrogens exerting endocrine effects on human hormone and metabolic signaling and may influence symptom penetrance in PCOS. We investigated whether women with PCOS and healthy controls have alterations in their capacity to metabolize hormonally active dietar...

Introduction: Diabetes mellitus (DM) may cause male infertility acting with pre-testicular, testicular and post-testicular mechanism.Aim: To evaluate the presence of male infertility and the mechanisms of sperm damage in diabetic patients in childbearing age.Patients and methods: Thirty-four patients with DM1, 55 with DM2 and 100 healthy fertile men (controls) were enrolled. Diabetic patients were further divided into three groups ...

Arterial hypertension usually results from the deregulation and hyperactivity of the renin angiotensin-aldosterone system. One of the available therapeutic approaches for its treatment is the use of mineralocorticoid receptor (MR) antagonists, thus blocking aldosterone action.The aim of this study was to characterize the effects of spironolactone and eplerenone, on the morphology of the adrenal gland of spontaneously hypertensive (SHR) and normotensive (...

Metabolic Syndrome (MetS) has been frequently associated with an overall inflammation status, called “metaflammation”, including atherosclerosis and non-alcholic steatohepatitis (NASH), In this study we aimed to evaluate the relationship between intra-prostatic inflammation and NASH in a cohort of patients affected by BPH. Between January 2012 and January 2016, 132 consecutive patients with BPH-related lower urinary tract symptoms (LUTS) who underwent transurethral r...

Introduction: Treatment of adrenal insufficiency (AI) in the last years has been object of important changes due to the development of a dual-release preparation of hydrocortisone (Plenadren®). Hydrocortisone dual-release therapy contemplates a once-daily tablet that allows more closely mimicking the physiological circadian rhythm cortisol, thus avoiding overexposure.Objective: The aim of the study was to value effects of Plenadren administration on...