Endocrine Abstracts

Annexin A1 (ANXA1), a Ca2+ and phospholipid binding protein, serves as a paracrine/justacrine mediator of glucocorticoid action in the neuroendocrine system. Within the anterior pituitary gland ANXA1 is produced by folliculo-stellate (FS) cells and, following serine-27 phosphorylation (Ser27-P) and membrane translocation, acts on adjoining corticotroph cells to suppress ACTH release via a mechanism involving formyl peptide receptor (FPR) activation. To ex...

Annexin 1 (ANXA1) is a 37 Kd member of the annexin family of Ca2+− and phospholipid binding proteins that has been well demonstrated to act as a mediator of glucocorticoid action in inflammation and in the control of anterior pituitary hormone release. ANXA1 is also proapoptotic and has been identified as a mediator of apoptotic cell engulfment (Solito et al. 2003 FASEB J 17:1544; Arur et al. 2003 Dev Cell 4:587). Using yeast two hybrid screening...

Annexin 1 (Anx-1), a 37kDa protein, is a member of the super-family of Ca 2+ and phospholipid-binding annexin proteins and acts as a mediator of glucocorticoid (GC) action in the host defense and neuroendocrine systems. In the anterior pituitary Anx-1 is expressed mainly by folliculostellate (FS) cells and mediates the early delayed feedback inhibition exerted by GCs on the release of ACTH and other pituitary hormones. It has been previously demonstrated that TtT/GF...

Improved knowledge of adipogenesis is necessary to gain insight into brown and white fat physiology. Interest in adipocyte differentiation has increased markedly over the past few years with emphasis on intersection between microRNAs (miRNAs) and the transcriptional cascade that controls adipogenesis and metabolic dysfunction. The aim of this study is to identify miRNAs that regulate white and brown adipocyte differentiation and define miRNA action in a stem cell model of adip...

Context: The polycystic ovarian syndrome (PCOS) is characterized by hyperandrogenism and associated with obesity and impaired glucose metabolism. Despite the high prevalence of PCOS and the considerable clinical impact, the precise interplay between metabolism and hyperandrogenemia is not entirely clear. We therefore aimed to analyse the relation between circulating free fatty acids (FFAs) and androgen metabolism in vivo in women.Design and partic...

Background: Brown adipose tissue (BAT) thermogenesis offers an appealing prospect to combat obesity. Obesity is characterised by a state of chronic inflammation in adipose tissue mediated by the secretion of a range of inflammatory-cytokines. Our previous work has highlighted that a gut-derived inflammatory agent, lipopolysaccharide (LPS), reduces brown adipocyte activity, insulin sensitivity and mitochondrial-function and is increased with obesity and type 2 diabetes mellitus...

The South Wales regional NET Multidisciplinary Meeting (MDT) discusses patients on a monthly basis. The European Neuroendocrine Tumour Society (ENETS) have guidance on expected pathology input into a NET MDT service. We assessed pathology reports submitted and discussed in 2016 for compliance with ENETS criteria, and determined the time from pathology reporting to MDT discussion. 117 patients had pathology submitted for review in 2016 and were assessed by one specialist NET pa...

Objective: To compare 3 methods of generating Ki-67% in gastroenteropancreatic neuroendocrine tumours (GEP-NETs).Methods: Twenty-four slides were used to generate 49 images for analysis. Each image was analysed and 3 different methods were used to calculate Ki-67%.Results: The comparison of Ki-67% from counting and estimating using the diameter (shortcut method) was strongly positively correlated, whereas the Ki-67% from counting a...

Choriogonadotrophin-alpha (Cga) is one of the first molecular markers for the developing pituitary gland previously known as ‘alpha-subunit’, common to thyroid-stimulating hormone (TSH), luteinising hormone (LH) and follicle-stimulating hormone (FSH). Mice lacking Cga are hypogonadal and exhibit profound hypothyroidism and dwarfism. Light microscopy of Cga null mice has shown that pituitary thyrotrophs...

Choriogonadotrophin-alpha (Cga) is one of the first molecular markers for the developing pituitary gland previously known as ‘alpha-subunit’, common to thyroid-stimulating hormone (TSH), luteinising hormone (LH) and follicle-stimulating hormone (FSH). Mice lacking Cga are hypogonadal and exhibit profound hypothyroidism and dwarfism. Light microscopy of Cga null mice has shown that pituitary thyrotrophs...