Endocrine Abstracts

Introduction: Type 1 Diabetes Mellitus (T1DM) causes autoimmune destruction of insulin producing b-cells, affecting approximately 8.4 million individuals worldwide. Autologous stem cell derived b cell (sc-b cell) transplantation is a promising treatment, however receipient immunological responses remain a challenge. This pilot study utilised mass cytometry (CyTOF) to assess immune responses in co-cultures of peripheral blood mononuclear cells (PBMC) with autol...

A 76-year-old lady with a background of Crohn’s disease (ileostomy in 1991) was referred to endocrinology in March 2022. Her 9-am cortisol level was <50 nmol/l, with a sodium of 130 mmol/l. These were done as she reported tiredness, lethargy and dizziness since November 2021. She had also lost 7 lb of weight. No evidence of hyperpigmentation. She had then been empirically started on hydrocortisone (10 mg-morning, 5 mg-afternoon and 5 mg-evening) pending evaluation of ...

Gas chromatography mass spectrometry (GC-MS) is the gold standard for urinary steroid profiling. Our established GC-MS method quantifies mineralocorticoids, glucocorticoids, and androgens. GC-MS assays require chemical derivatisation and long run times, rendering them unsuitable for high-throughput analysis. Our aim was to develop and validate a high-throughput urinary steroid profiling method using ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-LC...

Steroid profiling of biological fluids has been used for clinical and diagnostic research since the 1950s. Most methods focus on blood and urine, however, more recently saliva analysis has been employed as a non-invasive, simple to collect sample for the diagnosis of conditions such as Cushing’s and androgen excess. Despite its ease of collection, saliva remains an underutilised biofluid. To investigate the use of salivary steroid profiling for endocrine research, we aimed to ...

Background: GLP-1 agonism have the potential to affect gastric emptying (GE), yet the reports for subcutaneous semaglutide, currently the most effective GLP-1 RA approved for weight management, remain inconclusive. It has been demonstrated that semaglutide either had no effect on GE or it delayed GE only within the first hour, without late phase retention. Notably, those conclusions were made by an indirect method of estimation of GE through ingestion, absorption and determina...

Background: Development of multi-steroid profiling allows comprehensive investigation into the different branches of steroid metabolism. Immunoassays only allow analysis of a single steroid per assay and suffer from problems with specificity due to cross reactivity of similar steroids. Liquid-chromatography mass spectrometry has the specificity to analyse multiple steroids in a single experiment and the dynamic range to quantify steroids at high concentrations such as those ob...

The objective of the study was to assess the effect of intra-muscular testosterone on glycemic control, metabolic parameters and hypogonadal symptoms in men with hypogonadism and poorly controlled type 2 diabetes. This is a randomised double-blinded placebo-controlled add-on trial of intramuscular-testosterone undecanoate(Nebido®) administered every 12 weeks in 65 hypogonadal men with poorly-controlled diabetes. Phase-1 patients were randomly assigned to either...

The objective of the study was to assess the effect of intra-muscular testosterone on constitutional symptoms, sexual symptoms, memory and in men with hypogonadism and poorly-controlled type-2 diabetes. This is a randomised double-blinded placebo-controlled add-on trial of intramuscular testosterone undecanoate(Nebido®) administered every 12 weeks in 65 hypogonadal men with poorly-controlled diabetes. Phase-1 patients were randomly assigned to either treatment ...

Objective: The objective of the study was to assess the effect of intra-muscular testosterone on body composition in men with hypogonadism and poorly controlled type 2 diabetes.Research design and methods: This is a randomised double-blinded placebo-controlled add-on trial of intramuscular testosterone undecanoate administered every 12 weeks in 65 hypogonadal men with poorly-controlled diabetes. Phase 1 patients were randomly assigned to either treatment...

Background: Vitamin B12 (B12) deficiency results in disturbance of 1-carbon metabolites [methylmalonyl coenzyme A (MMA), homocysteine and S-adenosyl homocysteine (SAH), S-adenosyl methionine (SAM) and methionine] that collectively affect lipogenesis leading to risk of cardiovascular diseases. In clinical studies, B12 deficiency is associated with higher BMI and dyslipidaemia (high triglycerides and low HDL). The role of B12 in hepatic metabolism of...