Endocrine Abstracts

Inflamed ageing is associated with reduced muscle mass and increased susceptibility to TNF-induced muscle protein degradation. We therefore aimed to elucidate mechanisms supporting reduced hypertrophy and increased atrophy of skeletal myoblasts. C2 myoblasts display diminished differentiation and increased susceptibility to TNF-α-induced apoptosis versus daughter C2C12 cells, providing us with relevant models. C2 and C2</su...

Introduction: The role of TNF-alpha in muscle wasting diseases if a topic of great interest. However, the role of interleukin-6 (IL-6) in some pathological diseases and in skeletal muscle is controversial. Physical exercise provides protection against several human diseases, e.g. cardiovascular disease, heart failure and claudicatio intermittens. This protection is associated with the muscle specific production of IL-6. In contrast to its protective role during exercise, eleva...

A 54-year-old female presented to the neurologist in December 2017 with neuromuscular right arm weakness, lethargy and fascial swelling. She had a normal MRI head before being referred to the endocrine clinic with a TSH >125 mIU/l (0.27–4.2 mIU/l). Free T4 was 0.3 pmol/l (12.0–22.0) with a free T3 of <0.4 pmol/l (3.1–6.8). The thyroglobulin antibody was 1366 IU/ml (0–115) with a normal thyroid peroxidase level (TPO) 29.7 (0–34). No previous thy...

Objective: To evaluate the prevalence of the RET mutation and the genotype–phenotype relation in Saudi patients (families) with multiple endocrine neoplasia type 2A (MEN2A) or familial medullary thyroid carcinoma (FMTC).Design: Cross-sectional study.Patients and methods: A total of ten unrelated Saudi families with germline mutation of the RET protooncogene and/or immunohistochemistry diagnosis of MTC were identified. B...

This study aims to explore the baseline adipocytokine profiles of adult Saudis and evaluate their relationship in the development of insulin resistance. Three hundred adult Saudis with varying glucose tolerance were recruited. They were grouped into NGT, IGT and DM. Anthropometrics, glucose and lipid profiles were analyzed by routine methods; leptin, adiponectin, resistin and CRP were measured by ELISA. Insulin resistance was significantly correlated with levels of CRP (R</...

Tumour necrosis factor-alpha (TNF-α) is a pleiotropic cytokine well characterised as a mediator of skeletal muscle wasting. However, the role of interlukin-6 (IL-6) in skeletal muscle remains controversial.Objectives: We therefore investigated the potential interactive effects of TNF-α and IL-6 on murine C2 skeletal myoblast survival, differentiation and proliferation.Methods: Real time-PCR, creatine kinase assay, western...

Introduction: Prolonged elevations of proinflammatory cytokines are associated with sarcopenia muscle wasting (ageing) and in cancer cachexia. Increased activation of the IGF/insulin pathway is an attractive target for combating such wasting.Aims: Using rodent skeletal muscle cell lines we have investigated TNF-α/IGF-I interactions, in an attempt to mimic and understand mechanisms underlying skeletal muscle loss.Methods and re...

Objectives: Cardiovascular disease encountered the leading cause of death in thyroid cancer survivals exceeding death by cancer itself. Etiological factors are the therapy used and the duration of exposure to this it, the diagnosis of cancer itself, or the factors that led to this cancer. We aim to evaluate cardiometabolic risk factors in an Algerian cohort of thyroid cancer survivals.Methods: The study includes a cohort of patients followed in the thyro...

Numerous studies have identified several extra-skeletal health outcomes to be associated with vitamin D deficiency, yet a definitive causal link is yet to be discovered. Our recent 3D LC-nESI-FTMS proteomic analysis among normal and overweight but apparently healthy adult Saudis identified apolipoproteins, a known independent cardiovascular risk factor, as one of the serological molecular signatures that modulate vitamin D levels. The present interventional study aims to compa...

Nitric Oxide (NO) has been considered a very important molecule in the biological system because of its interaction with other molecules for mediating various bio-pathways. Its interaction with vitamin D in particular studied well in animal models, cell lines and patients with various diseases. However, the relationship between NO and vitamin D has never been studied in healthy adolescents. We aimed to determine the association of NO with vitamin D and other serological factor...